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Keywords Leishmania donovani;clone;expression; 杜氏利什曼原虫;克隆;表达;
Keywords Leishmania donovani;ITS;Gene cloning;Sequence analysis; 关键词利什曼原虫;内转录间隔区;基因克隆;序列分析;
Keywords Leishmania donovani;Amastin;Gene cloning;Expression; 杜氏利什曼原虫;无鞭毛体蛋白;基因克隆;表达;
Keywords Leishmania Donovani Internal transcribed spacer （ITS） Gene cloning Gene sequencing; 关键词杜氏利什曼原虫;可转录间隔区序列（ITS）;克隆;序列分析;
The parasite, a protozoan called Leishmania donovani, had become resistant to the drugs of choice, compounds based on the element antimony. 黑热病利什曼原虫这种原生动物，是造成利什曼病的寄生虫，已对原本使用的锑化合物药物产生抗药性。
CONSTRUCTION AND IMMUNE RESPONSE OF A DNA VACCINE VECTOR?EXPRESSING HEAT SHOCK PROTEIN 70 OF ?LEISHMANIA DONOVANI? 杜氏利什曼原虫热休克蛋白70的DNA疫苗表达载体构建及体内应答研究
Study on the Effect of Alginate Amphotericin B on Leishmania Donovani Prom astigotes in Vitro. 海藻酸钠两性霉素B对杜氏利什曼原虫前鞭毛体作用体外实验研究
The first discover S.suihominis , S . suihominis cases , Leishmania donovani and Kala-azar . 在广西第一次发现猪人肉孢子虫和猪人肉孢子虫病；
Under the same conditions, the Leishmania donovani transfer efficiency was lower than other Leishmania spp. 但相同的培养条件下，杜氏利什曼原虫转化效率低于其他种株利什曼原虫。
Visceral leishmaniasis (VL) is prevalent in more than 80 countries in Asia, Africa (Leishmania donovani), southern Europe (L. infantum) and southern America (L. chagasi). 摘要内脏利什曼病在亚洲、非洲、南欧、南美洲80多个国家广泛流行。
We amplified SSU rRNA of Leishmania donovani with primer R222 and R333 by reverse transcription polymerase chain reaction. 我们采用引物R222和R333建立杜氏利什曼原虫SSUrRNA逆转录多聚酶链反应（RT－PCR）。
A recombinant eukaryotic expression plasmid of amastin gene of Leishmania Donovani was successfully constructed,and can be expressed stably in the NIH3T3 cells. 成功地构建杜氏利什曼原虫无鞭毛体蛋白基因的真核表达重组质粒,并且该基因在NIH3T3细胞中获得了稳定表达。
CONCLUSION: A recombinant eukaryotic expression plasmid of amastin gene of Leishmania Donovani was successfully constructed,and can be expressed stably in the NIH3T3 cells. 结论:成功地构建杜氏利什曼原虫无鞭毛体蛋白基因的真核表达重组质粒,并且该基因在NIH3T3细胞中获得了稳定表达。
A chronic, often fatal disease occurring chiefly in Asia, caused by a protozoan parasite(Leishmania donovani) and characterized by irregular fever, enlargement of the spleen and liver, and emaciation. 黑热病一种主要流行于亚洲的致命的慢性疾病，由一种原生动物寄生虫引起（杜氏利什曼虫利什曼虫属），病状表现为不正常的发烧，脾和肝的肿大，以及消瘦
A chronic,often fatal disease occurring chiefly in Asia,caused by a protozoan parasite(Leishmania donovani) and characterized by irregular fever,enlargement of the spleen and liver,and emaciation. 黑热病，一种主要流行于亚洲的致命的慢性疾病，由一种原生动物寄生虫引起（杜氏利什曼虫利什曼虫属），病状表现为不正常的发烧，脾和肝的肿大，以及消瘦。
Infection by Leishmania donovani 杜[诺凡]氏利什曼原虫感染
Keywords Leishmania;Kala-azar;Promastigote;ELISA;Sero epidemiology; 利什曼原虫;黑热病;前鞭毛体抗原;ELISA法;血清流行病学;
Keywords Leishmania infantum;Asymptomatic infection;PCR; 婴儿利什曼原虫;无症状感染;PCR;
Keywords Leishmania;repetitive DNA sequence;identification; 利什曼原虫;DNA重复序列;虫株鉴定;
Keywords Leishmania;RACK DNA vaccine;T cell antigen;Gene cloning; 关键词利什曼原虫;激活蛋白激酶C受体;基因疫苗;T细胞抗原;基因克隆化;